To evaluate the allergenicity of rice flour in Korean atopic children, we performed the ELISA for rice specific IgE and igG4 using own product of partially purified crude extract of rice flour. Allergic skin test with 63 inhalant and food
antigens(Torii0 and rice extract was done by scratch method, Subject were divided into two groups ; Group I : subjects with positive skin reaction to buckwheat flour(n=13), Group II : subjects with negative skin reactio to buckwheat flour(n=14).
@ES The results were as follows ;
@EN 1. All subjects showed positive skin reactions to Dermatophagoides farinae(Df) and Dermatophagoides pternyssinus(Dp), and negative to wheat and rice flour antigens. The mean age, total serum IgE and skin reactions to Df and Dp were not
significantly
different between Group I and Group II.
2. The positive rates of rice specific IgE were 38.5% and 0% in Group I and Group II, respectively. The relative concentration of rice specific IgE in Group I and Group II were 1.1±0.83 and 0.4±0.37 and there was no significant difference
between
Group I and Group II.
3. The positive rates of rice specific igG4 were 92.3%(12 of 13) and 85.7%( 12 of 14), respectively. There was no significant difference in the relative concentration of rice specific IgG4 between Group I(2.6±0.45) and Group II(2.4±0.40).
4. The concentrations of specific IgE were not correlated with those of total serum IgE.
In conclusion, 5 out of the 27s subjects studied are considered as rice allergic patient by demonstration of rice specific IgE in their sera. The detection of rice specific IgG4 is a less specific diagnostic method in rice allergy, and it simply
represent the long term exposure of rice antigen.
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